产品货号：
GS4739
中文名称：
重组人TIGAR-TAT
英文名称：
Recombinant Human TP53-induced Glycolysis and Apoptosis Regulator-TAT
产品规格：
5μg
发货周期：
1～3天
产品价格：
询价
分子量：Approximately 31.7kDa,a single non-glycosylated polypeptide chain containing 283 amino acids.
储存条件：冷冻（-20℃）
概述
TIGAR is a p53-inducible enzyme that catalyzes the hydrolysis of fructose-2-6 bisphosphate(F-2-6-BP)to fructose-6-phosphate and inorganic phosphate.F-2-6-BP is a powerful activator of 6-phosphofructose-1 kinase,the rate limiting enzyme of glycolysis.By lowering the intracellular level of F-2-6-BP,TIGAR expression leads to increased glucose processing via the pentose phosphate pathway,the major cellular source for NADPH.Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other intracellular proteins into primary as well as transformed cells.Recombinant human TIGAR-TAT expressed in E.coli is a 31.7 kDa protein containing 283 amino-acid residues,including the 269 residues of full-length TIGAR fused to a 13-residue C-terminal peptide containing the TAT transduction domain(GGYGRKKRRQRRR).
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
相关搜索：重组人TIGAR-TAT，TIGAR-TAT, Human
储存条件：冷冻（-20℃）
概述
TIGAR is a p53-inducible enzyme that catalyzes the hydrolysis of fructose-2-6 bisphosphate(F-2-6-BP)to fructose-6-phosphate and inorganic phosphate.F-2-6-BP is a powerful activator of 6-phosphofructose-1 kinase,the rate limiting enzyme of glycolysis.By lowering the intracellular level of F-2-6-BP,TIGAR expression leads to increased glucose processing via the pentose phosphate pathway,the major cellular source for NADPH.Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other intracellular proteins into primary as well as transformed cells.Recombinant human TIGAR-TAT expressed in E.coli is a 31.7 kDa protein containing 283 amino-acid residues,including the 269 residues of full-length TIGAR fused to a 13-residue C-terminal peptide containing the TAT transduction domain(GGYGRKKRRQRRR).
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | ＞96%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Fully biologically active when compared to standard.The biological activity determined by its ability to protect U2OS cells from apoptosis induced by hydrogen peroxide is in a concentration range of 0.1-5.0μg/ml,after pretreating with rHuTIGAR-TAT for 4hours. |
| 成分 | Lyophilized from a 0.2μmfiltered concentrated solution in 20mMPB,pH 7.1,350 mM NaCl,10%Trehalose. |
| 内毒素 | Less than 0.1 EU/μg of rHuTIGAR-TAT as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml.Stock solutions should be apportioned into working aliquots and stored at ≤ -20?C.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | MARFALTVVR HGETRFNKEK IIQGQGVDEP LSETGFKQAA AAGIFLNNVK FTHAFSSDLM RTKQTMHGIL ERSKFCKDMT VKYDSRLRER KYGVVEGKAL SELRAMAKAA REECPVFTPP GGETLDQVKM RGIDFFEFLC QLILKEADQK EQFSQGSPSN CLETSLAEIF PLGKNHSSKV NSDSGIPGLA ASVLVVSHGA YMRSLFDYFL TDLKCSLPAT LSRSELMSVT PN |
相关搜索：重组人TIGAR-TAT，TIGAR-TAT, Human